Gray Line of Tennessee is proud to regularly serve the following enterprises:
- Belmont University
- Bridgestone Americas, Inc.
- Caterpillar Financial
- Dollar General Corporation
- Federal Emergency Management Agency
- Gaylord Opryland
- Hello! Nashville
- Hospital Corporation of America
- Middle Tennessee State University
- Nashville Predators
- Nashville Regional Transportation Authority
- Nashville SC
- Nashville Sounds
- NFL visiting teams
- NHL visiting teams
- The Simmons Bank Open
- State of Tennessee
- Tennessee State University
- Tennessee Titans
- US Department of Defense
- Vanderbilt University
- Western Kentucky University